SenNet Consortium Underrepresented Student Program (CUSP) Summer 2023
2024 applications are opening soon!
Meet the 2023 Students
Ms. Berhane is a recent graduate from Virginia Commonwealth University. She is interning at UCSD, where the lab is investigating senescent cells in aging and age related diseases in the liver, brain, bone marrow, breast, and colon across the lifespan of male and female mice. She will be focused on creating detailed single cell resolution maps of the transcriptome and epigenome in bone marrow, and assessing the effects of senolytics. Ms.Berhane aspires to be a Pediatric Hematologist/Oncologist serving underprivileged and marginalized communities, by reducing disparities in the diagnosis and treatment of hematological diseases.
My name is Kai Como, a senior, biology major at Prairie View A&M University. My internship is at Stanford University. My research team is in the radiology department. Our research aims to create a new biomarker that will detect senescent cells using the protein. I am writing a literature review on the expression of β-galactosidase in different tissues and organs. I will be conducting Western Blotting and PCR experiments on chondrocytes using cell cultures. I am most interested in the effects of senescent cells and β-galactosidase in the musculoskeletal system because I aspire to be an orthopedic surgeon.
My name is Cansu Eser; I am a rising junior at Dickinson College majoring in Biochemistry and Molecular Biology from Queens, NY. My internship is taking place at Cornell University in the Cosgrove Lab. I am currently learning the coding language R to analyze and score data collected regarding gene expression within cells in skeletal, muscle, and ovarian tissues to determine the likelihood of senescence. I am also shadowing grad students in the lab, learning how to perform dissection and organ collection on female mice at different points in their estrous cycle, and digest and harvest organoids from collected tissues.
My name is Kaia Gray, and I am a senior at Sam Houston State University. I am an intern at the University of Pittsburgh where research is being conducted on the cellular senescence of chronic lung diseases in aging individuals. This experience has given me the opportunity to work on various lab techniques and understand the ‘why’ of each step. I have been able to network and learn about different career paths in my time here. The lessons I have learned in this program have made me a better student, scientist, and person.
My name is Kamilah Islam, I am currently a rising senior at the University of Wisconsin Madison. I am studying Biology on a pre med track. This summer I am working as the SenNet Consortium intern. I have been working with Beth to do single cells RNAseq on both adipose and liver tissue. For adipose tissue I have been working with and cutting fresh and frozen tissue, in which I’ve done both nuclei isolation and RNA isolations. For liver tissue I have isolated RNA and fixed frozen tissue for single cell RNAseq. Future plans for the summer include qRT-PCR on liver and adipose tissue and SA-bgal staining with adipose tissue.
I’m Tanvi Jain, a rising Junior majoring in Bioinformatics at UC San Diego. I’m interning at Duke University this summer in data processing and visualization methods for sc Flow Cytometry data for the FlowKit package to highlight and analyze senescent cell types in lung and colon cell datasets in Python, along with learning the wet lab sc Flow Cytometry assays. I’m also experienced in scRNA-seq analysis as a part of HuBMAP, HLCA, and 1001 genomes project. I aspire to be working in entrepreneurial biomedical informatics and health-informatics.
Feel free to connect with me at www.linkedin.com/in/tanvi-jain-820271217
My name is Taejoon Kim, I am a rising senior, and I am a Macalester College student currently interning at the University of Minnesota. I am currently honing my PCR techniques so that I can start looking for senescence markers in high fat diet mice of different ages. I am most interested in the immune system and how it fends off a wide variety of pathogens/diseases.
My name is Maria Lewis and I’m a rising sophomore at Johns Hopkins University studying neuroscience with a double minor in Spanish and Psychology. I’m from Saint Paul, Minnesota and I’m currently doing research at Fan Lab at Yale University. I’m working with DBiT-seq, spatial transcriptomics, and staining to study senescent cells in human lymph tissues. My main goal of the summer is to learn new research techniques and enhance my understanding of senescent cells. Overall, I want to pursue a MD and I’m considering the MD/PhD path. I’m excited to have this opportunity and join my amazing team!
Thang "Mike" Mung
I am currently working at the Shu Lab at MGH. Under the tutelage of the research scientists, I have been gaining experience in single-cell techniques, Share-seq, and imaging instruments like the HT-1 microscope. In hopes of further understanding senescent cells in the human body, I hope to apply the knowledge and skills acquired to dive deeper into research in that area. In the near future, I hope to learn about Flow Cytometry and design my own experiment that will incorporate the skills I have learned. Ultimately, I hope to gain expertise in utilizing Raman microscopy to track single cell RNA profiles. I have had nothing but enjoyable experiences and comradery throughout my internship.
Ebenezer Nyenwe is a rising senior Bioinformatics major at Xavier University of Louisiana interested in using his studies to improve the overall quality of healthcare. He is currently at the Washington University site where he is learning about crucial lab procedures and the application of bioinformatics in modern medicine. He is interested in new, cutting-edge research that involves computational biology. He plans on building up his skills as a researcher and getting an MD/PhD.
Hello! My name is Fisayo Omonije, and I’m a rising sophomore at Johns Hopkins University. I’m currently interning at the Knight Cancer Research Lab at Oregon Health and Science University. In the lab, I have been collecting, cutting, and staining mouse tissue samples – to better understand the presence (and threat) of senescent cells in vivo.
I aspire to be an orthopedic specialist, which would allow me to combine my passion for medicine with my interest in sports science, as a current Division III track and cross-country athlete. I’m excited to continue to immerse myself in the biomedical sciences.
My name is Ji Seo, and I am currently a 4th year student at California State University San Marcos. My internship is at Ohio State University Medical Center, specifically at the Heart and Lung Research Institute. I am currently working on culture of lung cells and analysis of precision cut lung slices. These techniques will contribute to characterization of markers of senescence in different lung cell types. My ultimate goal is to attend medical school and receive my MD. I am looking forward to continually learn and grow from this wonderful experience as well as applying all of these advanced techniques for future research.
Requirements & Expectations
Accepted students are required to:
Conduct your own small research project or work on part of an ongoing research project.
Attend a weekly virtual seminar series that will introduce you to rapidly progressing medical and basic research areas.
Complete all Compliance Training, Conflict of Interest Forms and/or any other training deemed necessary for your internship as soon as it is received.
Report to work on time as designated by the mentor to work on assigned research project. Students are expected to work no more than 40 hours a week.
Present a virtual poster at the end of the project about your experience. The date of the presentation will be determined by the NIH SenNet program.
Successful applicants will have:
An interest in and passion for studying biological or computational sciences, or related STEM fields
At least a 2.5 GPA
A demonstrated strong work ethic
Some experience with lab work
A personal statement which outlines their interests and experience
Accepted students will receive a stipend, housing, and technology assistance as needed.
All applicants will be required to submit an application form, transcript, personal statement, and 2 letters of recommendation. All materials should be submitted via the application form, by March 31, 2023.
Transcript: Unofficial transcripts are acceptable. Please submit transcripts as an attachment to your application form, as a PDF.
Personal Statement: Please submit a personal statement as an attachment to your application form, briefly describing your background, interest in participating in this summer undergraduate research program in single cell science, and your career goals. Please attach your personal statement in PDF format. Personal statements should be no longer than 2 pages single spaced, font size 11, Times New Roman.
Letters of recommendation: Two letters of recommendation should be sent to the application committee from your references, by the application due date of March 31, 2023. Letters of recommendation should be sent to email@example.com
2023 CUSP Program Opportunities
University of California San Diego
Projects in my lab will focus on validation of genetic and pharmacological approaches to eliminate senescent cells from mouse liver, brain, mammary, colon and bone marrow
Columbia University Irving Medical Center
CUIMC tissue mapping center is building an atlas of senescence in healthy individuals from 20-80 years old. The incoming student will learn the visium workflow generating spatial sequencing data from postmortem human tissue. The workflow includes sectioning of cortex/hippocampus, H&E staining, quality control and library preparation.
Buck Institute for Research on Aging
Two potential projects- TMC (U54):
This project aims to identify, map and validate senescent cells in the human ovary and characterize cell types in the post-menopausal ovary. Trainees on this project will characterize the presence of canonical and novel senescence markers in human post-menopausal ovarian tissue using immunohistochemical and histological techniques. Then, using semi-quantitative analysis, they will map the distribution of markers in whole ovarian tissue sections across ages.
This project is piloting the use of the microphysiologic ex vivo tissue-on-a-chip platform (LATTICE) to study cellular senescence beyond standard static cell culture methods. LATTICE provides a more physiologic platform for screening and evaluating senolytics/senostatics and SASP immune-depletion. Trainees on this project will examine expression of canonical and novel senescence markers in doxorubicin-induced cellular senescence in a standard tissue culture system versus doxorubicin-induced senescence in culture using microphysiologic ex vivo tissue-on-a-chip platform (LATTICE).
Pacific Northwest National Laboratory or subaward Oregon Health & Science University
Our labs at the Pacific Northwest National Laboratory (PNNL) and Oregon Health & Science University (OHSU) are developing methods to identify and molecularly map senescent cells in tissues. We use a genetically-controlled, tamoxifen-inducible, senescent mouse model termed the FASST mouse to induce senescence stochastically in the stroma. One potential project for a CUSP trainee at OHSU would include histological characterization of FASST mouse tissue and would incorporate a variety of techniques including tissue isolation, embedding, fixation and immuno-histochemical staining. We are using the senescent tissue from this mouse to develop a spatial proteomics platform to characterize the senescence proteome and secretome. One potential CUSP project at PNNL would include proteomics characterization of FASST mouse tissue sections that would include laser microdissectioning, microfluidic sample preparation and liquid chromatography coupled with mass spectrometry.
Below are the couple of projects that will be feasible for the trainees during their time in our research group:
1. Immunohistochemistry of the pig knee sections to detect senescence.
2. Detecting senescence in human synoviocytes and chondrocytes derived from healthy individuals and those with osteoarthritis/rheumatoid arthritis using a novel radiotracer and validating it with conventional approaches.
3. Radiotracer based imaging of age-induced senescence across different healthy tissues in mouse models.
University of Minnesota – Bernlohr
The Midwest Mouse TMC invites students interested in summer internships in 2023 to consider opportunities to study cellular senescence within the context of adipose, muscle, lung, brain or liver biology. The MMTMC has opportunities to work alongside investigators assessing cellular senescence using state of the art omics technologies as applied to mouse models of aging. Work can either be focused on assessing protein or gene expression via a battery of technologies available to investigators as part of the MMTMC consortium.
University of Connecticut Health
1. Effects of senolytics (d+q) on beta cell function and gene identity using human islets from non-diabetic individuals
2. Relation between senescence on human pancreas vasculature and pancreatic islets
3. Morphometric distribution and analysis of pancreatic senescence using a p21cip-dTom mouse reporter model
The Washington University Senescence Tissue Mapping Center (WU-SN-TMC) is developing cellular senescence atlases with a focus on human liver and bone marrow tissues across the lifespan.
The candidate will work with an interdisciplinary team of investigators to support the construction of the cellular senescence atlases under investigation. Techniques learned may include tissue procurement and processing for high-dimensional genomics and proteomics analyses, computational analysis of multiple data types and/or validation of senescent biomarkers through in vitro cell and tissue culture experiments, depending on the interests of the candidate. The candidate will be and able to attend dynamic lab meetings and seminars and interact with graduate students, fellows, and senior scientists at an outstanding research environment.
The Duke University SenNet Tissue Mapping Center is developing novel statistical and computational methods for the analysis of single cell and spatial transcriptomics data sets from lung and colon samples. Potential summer projects for students with programming experience include 1) development of a dashboard for visualization of complex data sets, 2) exploring the chromatin accessibly landscape of senescent cells across normal tissues, and 3) application of text mining methods to build a knowledge graph of senescent cell biology. The intern would be working with a team of faculty, staff, postdocs and graduate students with expertise in statistics and computational biology, and get to take part in our dry lab meetings and journal clubs.
University of Michigan SenNet TDA is working on developing and optimizing Seq-Scope for sensitive detection and characterization of senescent cells in tissues. Seq-Scope is a novel technology that enables microscopic examination of spatial transcriptome in diverse tissues. Potential summer projects include (1) application of Seq-Scope to different tissues undergoing senescence, (2) optimization of Seq-Scope for detecting senescent cells, and (3) developing new functionalities in Seq-Scope.
University of Minnesota -Niedernhofer
Looking for 1 CUSP student this summer who is interested in bioinformatics and 1 interested in wet bench research.
Bioinformatics: Modeling senescent cell behavior and impact in human liver.
Wet bench: Cross-validation of senescent cell biomarkers across multiple human tissues and technology platforms (spatial transcriptomics and proteomics).
Yale University – Dixit
Project available to study effects of aging on murine spleen, lymph node and thymus and identification of key biomarkers defining aging. Studies for summer 2023 include advanced imaging of tissues.
Yale University – Fan
The study of cellular senescence in primary lymph tissues from younger and older human subjects.
The Fan lab has developed novel omics methods to study spatial expression of proteins, mRNAs and the underlying epigenetic mechanisms related to aging.
The Ohio State University
The trainee will be receiving training in the laboratory of Dr. Mauricio Rojas and Ana L Mora at the Davis Heart Research Lung Institute. The student will work directly under the supervision of postdoctoral fellow Drs. Lorena Rosas and Natalia Vanegas, learning the preparation, in vitro culture and analyses of the novel technique of precision cut lung slices. Studies will characterize markers of senescence using different stimuli and time points in the distinct lung cell types.
University of Pittsburgh
The Koenigshoff lab is focused on deciphering mechanisms involved in lung aging and regeneration, with the aim of identifying novel therapeutic targets relevant for age-related chronic lung diseases, such as idiopathic pulmonary fibrosis (IPF) and chronic obstructive pulmonary disease (COPD). The CUSP student will get introduced to human tissue derived models of lung aging and analyze senescence associated proteins. Major methodologies include cell and tissue culture as well as molecular biological analysis techniques. The student will get trained and work together with two TriState SenNet Postdoctoral Fellows in the laboratory and integrated in our weekly SenNet meetings. The Koenigshoff lab is composed of a multi-disciplinary group of people from different backgrounds and cultures with the guiding principle that diverse viewpoints and perspectives will lead to more creative and innovative, and thus better, science.
Cornell University (Ithaca, NY)
This project will involve developing and testing computational approaches to identify cellular senescence in single-cell multi-omic datasets collected from various mouse tissues. The researcher will perform bioinformatic programming to handle single-cell RNA-sequencing datasets and map gene expression signatures on to them.
Mayo – NIA SenNet Mouse (Mayo Clinic, Rochester MN)
This project will involve establishment of the location and identity of senescent cells from the mouse spinal cord in the Baker laboratory. Techniques that will be learned include animal handling, histology, RNA isolation, and gene expression profiling.
Massachusetts Institute of Technology
Applications Open: January 15, 2023
Applications Close: March 31, 2023
Internship Duration: 8-10 weeks between June 5 – August 18, 2023
Final Presentation: August 2023